Describe the synthesis pathway of the plant vacuole, including the roles of the ER and Golgi apparatus.

Proteins are synthesized in the cytosol and directly form the vacuole

Proteins are synthesized in the nucleus and transported to the vacuole

Proteins are synthesized in the ER, processed in the Golgi, and transported in vesicles to form the vacuole

Proteins are synthesized in the chloroplast and transported to the vacuole