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Microbiology Study Guide: Metabolism, Nutrition & Growth, Genetics, and Biotechnology

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Introduction to Energy

  • Thermodynamics: System vs. Surroundings
    04:21
  • Introduction to Energy
    04:58

ATP

  • Energy Coupling
    04:57
  • ATP
    06:44
  • Phosphorylation
    03:00

Introduction to Metabolism

  • Introduction to Metabolism
    02:17
  • Catabolic & Anabolic Pathways
    03:25

Redox Reactions

  • Redox Reactions Example 1
    01:44
  • Electron Carriers: NADH & FADH2
    05:22
  • Redox Reactions
    04:46

Types of Phosphorylation

  • Substrate-Level Phosphorylation
    03:00
  • Oxidative Phosphorylation
    06:27
  • Types of Phosphorylation
    01:06

Enzymes

  • Functions of Enzymes
    02:32
  • Enzymes Example 1
    02:25
  • Enzymes
    02:55

Enzyme Activation Energy

  • Enzyme Activation Energy
    04:18
  • Enzymes Lower EA
    03:26

Enzyme Binding Factors

  • Cofactors
    04:22
  • Enzyme-Substrate Complex
    03:49

Enzyme Inhibition

  • Enzyme Inhibition
    05:03

Negative & Positive Feedback

  • Positive Feedback
    02:19
  • Negative Feedback
    02:41

Introduction to Aerobic Cellular Respiration

  • Stages of Aerobic Cellular Respiration
    02:32
  • Introduction to Aerobic Cellular Respiration
    03:38
  • Introduction to Aerobic Cellular Respiration Example 2
    01:53

Glycolysis

  • Phases of Glycolysis
    05:57
  • Glycolysis Example 1
    01:29
  • Remembering Phases of Glycolysis
    02:49

Krebs Cycle

  • Krebs Cycle Example 1
    01:20
  • Krebs Cycle
    01:37
  • Phases of The Krebs Cycle
    09:54

Electron Transport Chain

  • Remembering the ETC
    04:43
  • Electron Transport Chain in Prokaryotes
    02:49
  • Electron Transport Chain
    07:41

Chemiosmosis

  • Chemiosmosis
    05:01

Review of Aerobic Cellular Respiration

  • Total Products for Each Stage of Aerobic Cellular Respiration
    07:25
  • Review of Aerobic Cellular Respiration
    07:50

Fermentation & Anaerobic Respiration

  • Lactic Acid Fermentation
    03:33
  • What Happens to Aerobic Organisms if There's No Oxygen?
    07:50
  • Alcohol Fermentation
    01:59

Microbial Growth Curves in a Closed System

  • Microbial Growth Curves in a Closed System
    08:59
  • Primary & Secondary Metabolites
    02:33

Temperature Requirements for Microbial Growth

  • Temperature Requirements for Microbial Growth
    04:16
  • Classification of Microbes by Growth Temperature
    04:56
  • How to Memorize the Classification of Microbes by Growth Temperatures
    01:59

Oxygen Requirements for Microbial Growth

  • Reactive Oxygen Species
    04:43
  • Oxygen Requirement Classes of Microbes
    07:27
  • Oxygen Requirements for Microbial Growth
    03:52

pH Requirements for Microbial Growth

  • pH Requirements for Microbial Growth
    03:11
  • Optimal Growth pH Classes of Microbes
    03:23

Osmolarity Factors for Microbial Growth

  • Osmolarity Factors for Microbial Growth
    06:30

Reviewing the Environmental Factors of Microbial Growth

  • Reviewing the Environmental Factors of Microbial Growth
    07:21

Nutritional Factors of Microbial Growth

  • Reviewing the Nutritional Growth Factors of Microbes
    02:20
  • Carbon Source:Heterotrophs vs. Autotrophs
    02:59
  • Nutritional Factors of Microbial Growth
    02:57

Growth Factors

  • Growth Factors
    01:55

Introduction to Cultivating Microbial Growth

  • Map of Techniques for Cultivating Microbes
    02:05
  • Introduction to Cultivating Microbial Growth
    03:49

Types of Solid Culture Media

  • Types of Solid Culture Media
    03:16

Plating Methods

  • Streak-Plate Method
    05:16
  • Spread-Plate Method vs. Pour-Plate Method
    05:36

Measuring Growth by Plate Counts

  • Measuring Growth by Plate Counts
    07:16
  • Measuring Growth by Plate Counts
    01:36

Measuring Growth by Membrane Filtration

  • Measuring Growth by Membrane Filtration
    02:29

Introduction to the Types of Culture Media

  • Introduction to the Types of Culture Media
    02:42

Selective Media

  • Selective Media
    03:27

Differential Media

  • Differential Media
    04:34

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Methods of Inducing Mutations

  • Chemical Mutagens
    05:13
  • Radiation Mutagens
    02:55
  • Methods of Inducing Mutations
    03:19

Mutant Detection

  • Mutant Detection
    06:49
  • Replica Plating
    05:36

The Ames Test

  • The Ames Test
    06:59

Introduction to DNA Repair

  • Introduction to DNA Repair
    02:47

DNA Repair Mechanisms

  • Nucleotide Excision Repair
    03:31
  • SOS Repair System
    03:50
  • Photoreactivation
    02:29

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

Conjugation: F Plasmids

  • F Plasmids
    05:57
  • Mechanism of F Plasmid Conjugation in E. coli
    04:12

Conjugation: Hfr & F' Cells

  • F’ Cell Conjugation
    04:31
  • Hfr Cell Conjugation
    09:41

Genome Variability

  • Phage DNA
    01:44
  • Genome Variability
    01:59
  • Genome Variability
    03:18

CRISPR CAS

  • CRISPR CAS
    07:43

Introduction to DNA-Based Technology

  • Map of DNA-Based Technology Lesson
    03:12
  • Introduction to DNA-Based Technology
    02:30

Introduction to DNA Cloning

  • Introduction to DNA Cloning
    02:25
  • Cloning with Recombinant DNA
    03:41

Steps to DNA Cloning

  • Steps to DNA Cloning
    04:42
  • 1b) Use Ligation Enzymes
    04:58
  • 1a) Use Restriction Enzymes
    03:27

Introduction to Polymerase Chain Reaction

  • Introduction to Polymerase Chain Reaction
    01:31
  • Components of a Polymerase Chain Reaction
    05:57
  • Why do we use PCR?
    02:53

The Steps of PCR

  • 1) Denaturation
    03:33
  • 2) Annealing
    02:56
  • The Steps of PCR
    04:39

Gel Electrophoresis

  • Gel Electrophoresis
    07:58
  • Gel Electrophoresis Example 1
    02:34

Southern Blotting

  • Southern Blotting
    03:31
  • Steps of Southern Blotting
    08:21

DNA Fingerprinting

  • Short Tandem Repeats (STRs)
    03:41
  • DNA Fingerprinting
    03:30

Introduction to DNA Sequencing

  • Introduction to DNA Sequencing
    02:13
  • Dideoxy Nucleotides
    05:12

Dideoxy Sequencing

  • Chain-Termination PCR
    07:37
  • Dideoxy Sequencing
    01:03
  • Determining the DNA Sequence from a Gel
    06:28