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Multiple Choice
In a blood sample collected for glucose measurement, which additive is commonly used to prevent glycolysis by inhibiting enolase?
A
Heparin
B
EDTA
C
Sodium fluoride (NaF)
D
Sodium citrate
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검증된 단계별 안내
1
Understand that glycolysis is the metabolic pathway that breaks down glucose, which can continue in a blood sample after collection, leading to decreased glucose levels and inaccurate measurements.
Recognize that to prevent glycolysis in blood samples, additives are used to inhibit enzymes involved in the glycolytic pathway.
Identify that enolase is one of the enzymes in glycolysis, and inhibiting it helps stop the breakdown of glucose.
Know that Sodium fluoride (NaF) is commonly used as an additive because it inhibits enolase, thereby preventing glycolysis and preserving glucose concentration in the sample.
Note that other additives like Heparin, EDTA, and Sodium citrate serve different purposes (e.g., anticoagulation) but do not effectively inhibit glycolysis by targeting enolase.