In the glycolytic pathway, how many molecules of NADH are produced per molecule of glucose converted to pyruvate?
A
NADH
B
NADH
C
NADH
D
NADH
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1
Recall that glycolysis is the metabolic pathway that converts one molecule of glucose into two molecules of pyruvate.
Understand that during glycolysis, specific enzymatic steps involve the reduction of NAD+ to NADH, which is a key electron carrier.
Identify the step in glycolysis where NAD+ is reduced: this occurs during the conversion of glyceraldehyde-3-phosphate (G3P) to 1,3-bisphosphoglycerate, catalyzed by glyceraldehyde-3-phosphate dehydrogenase.
Since one glucose molecule produces two molecules of G3P, and each G3P produces one NADH, multiply the number of NADH produced per G3P by 2 to account for both molecules.
Conclude that the total NADH produced per glucose molecule during glycolysis is the product of NADH per G3P times 2.