In aerobic glycolysis of one molecule of glucose to two molecules of pyruvate, what is the net production of ATP (accounting for ATP used and produced by substrate-level phosphorylation)?
A
ATP (net)
B
ATP (net)
C
ATP (net)
D
ATP (net)
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1
Identify the total number of ATP molecules consumed during the initial steps of glycolysis. Specifically, note that ATP is used in the phosphorylation of glucose and fructose-6-phosphate.
Determine the total number of ATP molecules produced during the later steps of glycolysis, particularly during substrate-level phosphorylation when 1,3-bisphosphoglycerate and phosphoenolpyruvate are converted to 3-phosphoglycerate and pyruvate, respectively.
Remember that glycolysis converts one molecule of glucose into two molecules of pyruvate, so multiply the ATP produced per molecule of substrate by 2 to account for both pyruvate molecules formed.
Calculate the net ATP production by subtracting the ATP consumed from the ATP produced: use the formula \(\text{Net ATP} = \text{ATP produced} - \text{ATP consumed}\).
Conclude the net ATP yield from glycolysis, which reflects the balance of ATP molecules used and generated through substrate-level phosphorylation.