why-is-it-challenging-to-determine-the-order-of-peptide-fragments-in-the-origina

Question

Why is it challenging to determine the order of peptide fragments in the original protein sequence after cleavage?

Accepted Answer

Cleavage can produce fragments of similar size, making it difficult to determine their order.

Answer

The original sequence is lost during the cleavage process.

Answer

Cleavage reagents often modify the amino acids, altering the sequence.

Answer

Fragments are often degraded before sequencing can occur.