How does Le Chatelier's principle explain the effect of uncompetitive inhibitors on enzyme affinity?

The decrease in enzyme-substrate complex concentration shifts the equilibrium to the right, increasing enzyme affinity for the substrate.

The equilibrium shifts to the left, increasing enzyme affinity for the substrate.

The equilibrium remains unchanged, so enzyme affinity is unaffected.

The increase in enzyme-substrate complex concentration shifts the equilibrium to the left, decreasing enzyme affinity for the substrate.