why-is-iodoacetate-used-in-conjunction-with-beta-mercaptoethanol-during-protein-

Question

Why is iodoacetate used in conjunction with beta mercaptoethanol during protein sequencing?

Accepted Answer

To prevent the reformation of disulfide bonds by carboxymethylating sulfhydryl groups.

Answer

To enhance the cleavage of peptide bonds.

Answer

To label the N-terminal amino acids.

Answer

To cleave peptide bonds at the C-terminal end.