why-is-blocking-unoccupied-sites-with-non-specific-proteins-crucial-in-elisa

Question

Why is blocking unoccupied sites with non-specific proteins crucial in ELISA?

Accepted Answer

To prevent non-specific binding of antibodies, ensuring accurate results.

Answer

To increase the binding affinity of the secondary antibody.

Answer

To facilitate the removal of unbound antigens during washing steps.

Answer

To enhance the enzymatic activity of the secondary antibody.