if-a-mixed-inhibitor-has-a-ki-of-5-m-and-a-ki-of-10-m-what-does-this-indicate-ab

Question

If a mixed inhibitor has a Ki of 5 µM and a Ki' of 10 µM, what does this indicate about its binding affinity?

Accepted Answer

The inhibitor has a higher affinity for the free enzyme than for the enzyme-substrate complex.

Answer

The inhibitor binds equally to the free enzyme and the enzyme-substrate complex.

Answer

The inhibitor has a higher affinity for the enzyme-substrate complex than for the free enzyme.

Answer

The inhibitor does not bind to either the free enzyme or the enzyme-substrate complex.