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Titration quiz

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  • What is the primary purpose of a titration in biochemistry labs?
    Titrations are used to measure pH changes and determine the pKa values and concentrations of acids, especially weak acids, in biological systems.
  • What is the titrant in a titration experiment?
    The titrant is the solution of known concentration that is gradually added to the analyte solution during a titration.
  • What is the analyte in a titration experiment?
    The analyte is the solution of unknown concentration being analyzed in a titration.
  • How is the equivalence point visually indicated during a titration?
    The equivalence point is indicated by a color change in the solution, signaling that neutralization has occurred.
  • What happens at the equivalence point in a titration?
    At the equivalence point, the moles of titrant added are exactly equal to the moles of analyte present.
  • What is plotted on the axes of a titration curve?
    The y-axis shows the pH of the analyte solution, and the x-axis shows the amount of titrant added.
  • What is the pH at the equivalence point for a strong acid-strong base titration?
    The equivalence point for a strong acid-strong base titration occurs at pH 7.
  • How can you identify a strong acid-strong base titration from its titration curve?
    The curve starts with a very low pH, rises sharply, and the equivalence point is at pH 7.
  • What is the inflection point (midpoint) on a titration curve of a weak acid?
    The inflection point is where half of the acid is neutralized, and the pH equals the pKa of the weak acid.
  • What is true about the equivalence point pH in a weak acid-strong base titration?
    The equivalence point pH is above 7, typically corresponding to the pKa of the weak acid.
  • What does the inflection point indicate in a polyprotic acid titration?
    Each inflection point indicates the pKa value of one of the acidic hydrogens in the polyprotic acid.
  • How many equivalence points and inflection points does a polyprotic acid with three acidic hydrogens have?
    It has three equivalence points and three inflection points, one for each acidic hydrogen.
  • What are the pKa values for the three acidic hydrogens in phosphoric acid (H3PO4)?
    The pKa values are 2.2, 7.2, and 12.7 for the three acidic hydrogens in phosphoric acid.
  • At each inflection point in a polyprotic acid titration, what is true about the concentrations of conjugate acid and base?
    At each inflection point, the concentration of conjugate acid equals the concentration of conjugate base.
  • What happens at the first equivalence point in the titration of phosphoric acid?
    At the first equivalence point, H3PO4 is completely neutralized and H2PO4- is at its maximum concentration.