Question 1

Which enzyme catalyzes the conversion of dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (G3P) in glycolysis?

Accepted Answer

Triose phosphate isomerase

Question 2

What is the net change in ATP molecules during the reaction catalyzed by phosphoglycerate kinase in glycolysis?

Accepted Answer

$$\Delta ATP = +1$$

Question 3

During glycolysis, which molecule is produced when 1,3-bisphosphoglycerate is converted by phosphoglycerate kinase?

Accepted Answer

3-phosphoglycerate

Question 4

Which of the following reactions in glycolysis involves the reduction of NAD$$^+ to $$NADH?

Accepted Answer

Glyceraldehyde-3-phosphate dehydrogenase

Question 5

If glycolysis is proceeding with one molecule of glucose, how many molecules of NADH are produced by the action of glyceraldehyde-3-phosphate dehydrogenase?

Accepted Answer

2

Question 6

Which step in glycolysis is directly responsible for substrate-level phosphorylation?

Accepted Answer

Phosphoglycerate kinase

Question 7

What is the main function of phosphoglycerate mutase in glycolysis?

Accepted Answer

Converts 3-phosphoglycerate to 2-phosphoglycerate

Question 8

Which of the following best describes the energy change ($$\Delta G^\circ') $$for the triose phosphate isomerase reaction?

Accepted Answer

$$\Delta G^\circ' = +7.5 kJ/$$mol

Question 9

In the reaction catalyzed by glyceraldehyde-3-phosphate dehydrogenase, which molecule acts as the electron acceptor?

Accepted Answer

NAD$$^+$$

Question 10

A patient has a deficiency in phosphoglycerate kinase. Which of the following would most likely be observed in their red blood cells?

Accepted Answer

Decreased levels of 3-phosphoglycerate

Question 11

Which glycolytic intermediate is formed immediately after the action of phosphoglycerate mutase?

Accepted Answer

2-phosphoglycerate

Question 12

Which of the following reactions in glycolysis is considered reversible under physiological conditions?

Accepted Answer

Triose phosphate isomerase

Question 13

During glycolysis, which step involves the transfer of a phosphate group from a high-energy intermediate to ADP?

Accepted Answer

Phosphoglycerate kinase

Question 14

Which glycolytic enzyme is responsible for the interconversion of DHAP and G3P?

Accepted Answer

Triose phosphate isomerase

Question 15

If a mutation caused the triose phosphate isomerase enzyme to lose function, which glycolytic intermediate would accumulate?

Accepted Answer

DHAP

Question 16

Which glycolytic reaction produces ATP by substrate-level phosphorylation?

Accepted Answer

Phosphoglycerate kinase

Question 17

What is the role of NAD$$^+ in $$the reaction catalyzed by glyceraldehyde-3-phosphate dehydrogenase?

Accepted Answer

Accepts electrons to become NADH

Question 18

Which glycolytic enzyme catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate?

Accepted Answer

Phosphoglycerate mutase

Question 19

A patient presents with a genetic mutation that impairs the function of glyceraldehyde-3-phosphate dehydrogenase. Which of the following would most likely be decreased in their cells?

Accepted Answer

NADH

Question 20

Which glycolytic intermediate is a substrate for phosphoglycerate kinase?

Accepted Answer

1,3-bisphosphoglycerate

Glycolysis: Key Steps and Mechanisms

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