Q: Which of the following reactions in glycolysis requires ATP as a substrate?

$$\text{glucose} \rightarrow \text{glucose-6-phosphate}$$

Q: Which of the following reactions in glycolysis produces ATP as a product?

$$\text{phosphoenolpyruvate} \rightarrow \text{pyruvate}$$

Q: Which of the following is involved in lactic acid fermentation?

$$\text{pyruvate} \rightarrow \text{lactate}$$

Q: In glycolysis, which step is considered the committed step?

$$\text{fructose-6-phosphate} \rightarrow \text{fructose-1,6-bisphosphate}$$

Q: Which of the following steps in glycolysis is irreversible under physiological conditions?

$$\text{glucose} \rightarrow \text{glucose-6-phosphate}$$

Q: Which step in glycolysis involves the oxidation of a substrate and reduction of NAD$$^+$$?

$$\text{glyceraldehyde-3-phosphate} \rightarrow \text{1,3-bisphosphoglycerate}$$

Q: Which of the following is a regulatory step in glycolysis?

$$\text{fructose-6-phosphate} \rightarrow \text{fructose-1,6-bisphosphate}$$

Question 1

During glycolysis, the conversion of 1 mole of fructose-1,6-bisphosphate to 2 moles of pyruvate results in which of the following net products?

Accepted Answer

2 moles NADH and 2 moles ATP

Question 2

If glucose labeled with $$^{14}C in C-3 $$and C-4 gives rise to pyruvate labeled in:

Accepted Answer

the carboxyl carbon

Question 3

If glucose was labeled with $$^{14}C at C-1 $$and fed to yeast carrying out the ethanol fermentation, where would the $$^{14}C$$ label be in the product?

Accepted Answer

C-1 of ethanol and CO$$_2$$

Question 4

Which of the following compounds is an intermediate of glycolysis?

Accepted Answer

Fructose-1,6-bisphosphate

Question 5

Which of the following reactions in glycolysis requires ATP as a substrate?

Accepted Answer

$$	ext{glucose} ightarrow 	ext{glucose-6-phosphate}$$

Question 6

Which of the following reactions in glycolysis produces ATP as a product?

Accepted Answer

$$	ext{phosphoenolpyruvate} ightarrow 	ext{pyruvate}$$

Question 7

Which of the following is involved in lactic acid fermentation?

Accepted Answer

$$	ext{pyruvate} ightarrow 	ext{lactate}$$

Question 8

Which enzyme catalyzes the conversion of phosphoenolpyruvate to pyruvate in glycolysis?

Accepted Answer

Pyruvate kinase

Question 9

In glycolysis, which step is considered the committed step?

Accepted Answer

$$	ext{fructose-6-phosphate} ightarrow 	ext{fructose-1,6-bisphosphate}$$

Question 10

Which of the following is a net result of glycolysis from one molecule of glucose?

Accepted Answer

2 ATP, 2 NADH, and 2 pyruvate

Question 11

Which glycolytic intermediate is directly involved in substrate-level phosphorylation?

Accepted Answer

1,3-bisphosphoglycerate

Question 12

Which enzyme is responsible for the phosphorylation of glucose in the first step of glycolysis?

Accepted Answer

Hexokinase

Question 13

During anaerobic glycolysis in muscle cells, what is the fate of pyruvate?

Accepted Answer

It is reduced to lactate

Question 14

Which of the following steps in glycolysis is irreversible under physiological conditions?

Accepted Answer

$$	ext{glucose} ightarrow 	ext{glucose-6-phosphate}$$

Question 15

Which glycolytic enzyme is allosterically inhibited by ATP?

Accepted Answer

Phosphofructokinase

Question 16

In alcoholic fermentation, which enzyme catalyzes the conversion of pyruvate to acetaldehyde?

Accepted Answer

Pyruvate decarboxylase

Question 17

Which of the following is NOT a product of glycolysis?

Accepted Answer

CO$$_2$$

Question 18

Which glycolytic intermediate is split to form two three-carbon molecules?

Accepted Answer

Fructose-1,6-bisphosphate

Question 19

Which step in glycolysis involves the oxidation of a substrate and reduction of NAD$$^+$$?

Accepted Answer

$$	ext{glyceraldehyde-3-phosphate} ightarrow 	ext{1,3-bisphosphoglycerate}$$

Question 20

Which of the following is a regulatory step in glycolysis?

Accepted Answer

$$	ext{fructose-6-phosphate} ightarrow 	ext{fructose-1,6-bisphosphate}$$

Glycolysis: Practice Questions and Key Concepts

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