Question 1

Which enzyme is primarily responsible for the conversion of glucose to glucose-6-phosphate in the first step of glycolysis?

Accepted Answer

Hexokinase

Question 2

During glycolysis, which molecule is directly phosphorylated by phosphofructokinase?

Accepted Answer

Fructose-6-phosphate

Question 3

Which of the following reactions in glycolysis produces ATP?

Accepted Answer

Phosphoglycerate kinase reaction

Question 4

What is the net ATP yield from one molecule of glucose during glycolysis?

Accepted Answer

$$2$$

Question 5

Which enzyme catalyzes the conversion of phosphoenolpyruvate to pyruvate?

Accepted Answer

Pyruvate kinase

Question 6

Which of the following is a substrate for the enzyme phosphoglycerate kinase?

Accepted Answer

1,3-bisphosphoglycerate

Question 7

Which glycolytic intermediate is formed by the action of aldolase?

Accepted Answer

Glyceraldehyde-3-phosphate

Question 8

Which enzyme is responsible for the isomerization of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate?

Accepted Answer

Triose phosphate isomerase

Question 9

Which of the following enzymes is NOT involved in the phosphorylation of glucose or its derivatives during glycolysis?

Accepted Answer

Aldolase

Question 10

A patient presents with a deficiency in phosphofructokinase. Which step of glycolysis would be directly affected?

Accepted Answer

Conversion of fructose-6-phosphate to fructose-1,6-bisphosphate

Question 11

Which glycolytic enzyme is regulated by ATP as an allosteric inhibitor?

Accepted Answer

Phosphofructokinase

Question 12

Which of the following is the correct order of glycolytic intermediates from glucose to pyruvate?

Accepted Answer

Glucose → Fructose-6-phosphate → Fructose-1,6-bisphosphate → Glyceraldehyde-3-phosphate → 1,3-bisphosphoglycerate → 3-phosphoglycerate → 2-phosphoglycerate → Phosphoenolpyruvate → Pyruvate

Question 13

Which enzyme catalyzes the transfer of a phosphate group from ATP to glucose?

Accepted Answer

Hexokinase

Question 14

Which glycolytic enzyme is responsible for the conversion of 3-phosphoglycerate to 2-phosphoglycerate?

Accepted Answer

Phosphoglycerate mutase

Question 15

In a metabolic disorder where enolase is deficient, which glycolytic intermediate would accumulate?

Accepted Answer

2-phosphoglycerate

Question 16

Which of the following enzymes is involved in substrate-level phosphorylation during glycolysis?

Accepted Answer

Phosphoglycerate kinase

Question 17

Which glycolytic enzyme is most likely to be regulated by feedback inhibition in muscle cells during intense exercise?

Accepted Answer

Phosphofructokinase

Question 18

Which of the following best describes the role of triose phosphate isomerase in glycolysis?

Accepted Answer

It converts dihydroxyacetone phosphate to glyceraldehyde-3-phosphate

Question 19

Which glycolytic enzyme is responsible for the final step of glycolysis, producing pyruvate?

Accepted Answer

Pyruvate kinase

Overview of Phosphorylation and Phosphate-Containing Biomolecules

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