Q: Which of the following equations correctly calculates the total magnification of a compound light microscope?

$$\text{Total Magnification} = \text{Ocular Lens Magnification} \times \text{Objective Lens Magnification}$$

Q: A student is asked to convert 0.25 kilometers to centimeters. What is the correct answer?

$$0.25 \text{ km} = 25,000 \text{ cm}$$

Question 1

Which of the following best describes the function of the coarse adjustment knob on a compound light microscope?

Accepted Answer

It is used to bring the specimen into rough focus by moving the stage up and down.

Question 2

A student is testing the effect of temperature on enzyme activity. She keeps pH and substrate concentration constant, but varies the temperature. Which is the independent variable in this experiment?

Accepted Answer

Temperature

Question 3

Which of the following statements about the Bradford protein assay is correct?

Accepted Answer

The assay produces a blue color in the presence of protein, and the intensity of the color is proportional to protein concentration.

Question 4

A dialysis bag containing starch and glucose is placed in a beaker of iodine solution. After 30 minutes, the solution inside the bag turns blue-black, but the beaker solution tests positive for glucose. Which molecule(s) diffused across the dialysis tubing?

Accepted Answer

Only glucose and iodine

Question 5

Which of the following best defines a scientific theory?

Accepted Answer

A well-supported explanation of natural phenomena that is based on a large body of evidence.

Question 6

A solution is tested with Benedict's reagent and heated. The solution turns orange. What does this indicate?

Accepted Answer

The presence of a reducing sugar.

Question 7

Which of the following equations correctly calculates the total magnification of a compound light microscope?

Accepted Answer

$$	ext{Total Magnification} = 	ext{Ocular Lens Magnification} 	imes 	ext{Objective Lens Magnification}$$

Question 8

A plant cell is placed in a hypotonic solution. What will most likely happen to the cell?

Accepted Answer

It will become turgid as water enters the cell.

Question 9

Which of the following is the correct order of steps in the modern scientific method?

Accepted Answer

Observation, hypothesis, experiment, data collection, analysis, conclusion

Question 10

Which macromolecule is correctly paired with its monomer?

Accepted Answer

Protein – amino acid

Question 11

A student uses chromatography to separate plant pigments. She measures the distance the pigment traveled as 2.5 cm and the distance the solvent front traveled as 5.0 cm. What is the $$R_f$$ value for this pigment?

Accepted Answer

$$R_f = \frac{2.5}{5.0} = 0.5$$

Question 12

Which of the following best describes the function of bromothymol blue in a cell respiration experiment?

Accepted Answer

It is a pH indicator that turns yellow in acidic conditions and blue in basic conditions.

Question 13

Which of the following factors does NOT affect the rate of diffusion across a membrane?

Accepted Answer

Color of the solute

Question 14

A student is asked to convert 0.25 kilometers to centimeters. What is the correct answer?

Accepted Answer

$$0.25 	ext{ km} = 25,000 	ext{ cm}$$

Question 15

Which of the following is an example of a controlled variable in an experiment testing the effect of light intensity on photosynthesis rate?

Accepted Answer

Temperature of the environment

Question 16

Which test would you use to detect the presence of starch in a food sample, and what color change would indicate a positive result?

Accepted Answer

Iodine test; blue-black color

Question 17

Which of the following best describes the difference between qualitative and quantitative data?

Accepted Answer

Qualitative data describes characteristics; quantitative data involves numerical measurements.

Question 18

Which of the following is NOT considered personal protective equipment (PPE) in a biology laboratory?

Accepted Answer

Open-toed sandals

Question 19

Which organelle is responsible for energy production in animal cells?

Accepted Answer

Mitochondrion

Question 20

During cellular respiration, which of the following is a substrate and which is a product?

Accepted Answer

Glucose is a substrate; carbon dioxide is a product.

General Biology Lab Study Guide: Safety, Scientific Method, Microscopy, Macromolecules, Diffusion, and Core Biochemical Processes

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