$$\text{Total Magnification} = \text{Eyepiece Magnification} \times \text{Objective Magnification}$$

Question 1

Which of the following best describes the difference between magnification and resolution in microscopy?

Accepted Answer

Magnification refers to how large an image appears, while resolution refers to the ability to distinguish two points as separate.

Question 2

A student is using a compound light microscope with a 10x eyepiece and a 40x objective lens. What is the total magnification?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

Why is it important to start focusing with the lowest power objective lens when using a compound light microscope?

Accepted Answer

It allows you to locate and center the specimen more easily before increasing magnification.

Question 4

Which part of the microscope is responsible for adjusting the amount of light that reaches the specimen and can also impact contrast?

Accepted Answer

Iris diaphragm

Question 5

A student observes that their specimen appears very faint and lacks detail. Which two actions could improve the contrast?

Accepted Answer

Adjust the iris diaphragm and stain the specimen.

Question 6

If the field of view under the scanning objective (4x) is 4600 µm, what is the field of view under the high power objective (40x)?

Accepted Answer

$$\frac{4600\ \mu m}{10} = 460\ \mu m$$

Question 7

Which cleaning material should always be used to clean microscope lenses?

Accepted Answer

Lens paper

Question 8

What is the correct order for putting away a compound light microscope after use?

Accepted Answer

Turn to 4x objective, lower the stage, remove the slide

Question 9

Why should you only use the fine adjustment knob when focusing with the 40x objective lens?

Accepted Answer

Using the coarse adjustment knob at high magnification can damage the slide or lens.

Question 10

A student is viewing a slide and wants to see all layers of a thick specimen in focus at once. Which feature should they adjust?

Accepted Answer

Increase the depth of focus

Question 11

Which of the following best explains why increasing magnification without increasing resolution is not useful?

Accepted Answer

The image will appear larger but will remain blurry and details will not be distinguishable.

Question 12

Which formula correctly calculates the total magnification of a compound light microscope?

Accepted Answer

$$	ext{Total Magnification} = 	ext{Eyepiece Magnification} 	imes 	ext{Objective Magnification}$$

Question 13

Which scenario best illustrates the concept of 'parfocal' in a microscope?

Accepted Answer

When switching from low to high power, the specimen remains nearly in focus.

Question 14

A student is preparing a wet mount slide and accidentally uses Kimwipes to clean the objective lens. What is the most likely consequence?

Accepted Answer

The lens may become scratched, reducing image quality.

Question 15

Which of the following is NOT a correct safety or care procedure when handling a compound light microscope?

Accepted Answer

Scooting the microscope across the bench top to move it.

Question 16

If you want to estimate the size of a specimen under the microscope, which information do you need?

Accepted Answer

The diameter of the field of view at the current magnification.

Question 17

Which of the following best describes the function of the condenser in a compound light microscope?

Accepted Answer

It focuses light onto the specimen.

Question 18

A student is viewing a stained cheek cell under the microscope. What is the primary reason for staining the specimen?

Accepted Answer

To improve the contrast and make cell structures more visible.

Question 19

Which step should be performed FIRST when beginning to use a compound light microscope?

Accepted Answer

Start with the stage all the way down and the 4x objective in place.

Question 20

Which of the following best explains why the field of view decreases as magnification increases?

Accepted Answer

Higher magnification lenses have a smaller diameter, so you see a smaller area of the specimen.

The Compound Light Microscope: Principles and Practice

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