which-step-in-the-sanger-sequencing-process-involves-the-use-of-pcr-and-what-is-

Question

Which step in the Sanger sequencing process involves the use of PCR, and what is its purpose?

Accepted Answer

DNA amplification to create multiple copies of the DNA fragment.

Answer

Separation of DNA fragments by size using gel electrophoresis.

Answer

Incorporation of ddNTPs to terminate DNA strand elongation.

Answer

Labeling of DNA fragments with fluorescent dyes.