Question 1

Which product is produced by the regulator gene of the lac operon in E. coli?

Accepted Answer

Repressor

Question 2

In the lac operon of E. coli, what role does lactose (or allolactose) play?

Accepted Answer

Inducer

Question 3

A lac operon merozygote with genotype $$I^p O^+ Z_1$^+ / I^p O^c Z_2$^+ is $$grown in an environment without lactose. Which z gene(s) will be transcribed?

Accepted Answer

Neither $$z_1$$ nor $$z_2$$ will be transcribed

Question 4

A lac operon merozygote with genotype $$I^p O^+ Z_1$^+ / I^p O^c Z_2$^+ is $$grown in an environment with lactose. Which z gene(s) will be transcribed?

Accepted Answer

Both $$z_1$$ and $$z_2$$ will be transcribed

Question 5

An $$O^c$$ mutation in the lac operon is best described as:

Accepted Answer

Two of these choices

Question 6

Catabolite repression in E. coli involves which of the following?

Accepted Answer

Cyclic-AMP levels

Question 7

Which of the following DNA sequences is/are likely to be recognized by a type II restriction endonuclease in double-stranded form?

Accepted Answer

Two of these choices

Question 8

A plasmid containing a foreign DNA insert is known as:

Accepted Answer

Two of these choices

Question 9

Which locus is commonly used for selecting hybrid vectors in DNA cloning experiments?

Accepted Answer

Two of these choices

Question 10

DNA cloning can be performed on blunt-ended DNA using which of the following techniques?

Accepted Answer

All of these choices

Question 11

What is the key enzyme required for blunt-end ligation in DNA cloning?

Accepted Answer

T4 DNA ligase

Question 12

The form of DNA called cDNA refers to:

Accepted Answer

Complementary DNA

Question 13

Which laboratory method is used to transfer electrophoresed DNA to a membrane for hybridization?

Accepted Answer

Southern blotting

Question 14

Plasmids that allow both transcription and translation of their cloned genes are called:

Accepted Answer

Expression vectors

Question 15

The technique of studying an extended length of DNA by way of overlapping clones is called:

Accepted Answer

Chromosome walking

Question 16

For a prokaryotic plasmid to grow successfully in yeast, it must contain which element?

Accepted Answer

CEN

Question 17

Which amplification technique is used for DNA lying between known sequences?

Accepted Answer

PCR

Question 18

When several loci control a phenotype and each locus has an allele that contributes an equivalent small increment to that phenotype, the inheritance pattern is called:

Accepted Answer

Additive

Question 19

Traits exhibiting quantitative inheritance are called:

Accepted Answer

All of these choices

Question 20

Polygenes are typically located:

Accepted Answer

On all chromosomes

Genetics Final Exam Study Guide: Gene Regulation, DNA Technology, and Quantitative Genetics

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