In the plaque assay, exactly what makes up a single plaque?

Name: Essentials of Genetics
Author: Klug
ISBN: 9780135588789

검증된 단계별 안내

Understand that a plaque assay is a method used to quantify the number of virus particles capable of infecting cells in a sample.

Recognize that a single plaque represents a clear zone on a layer of host cells where the virus has infected and lysed the cells.

Know that each plaque originates from a single infectious virus particle, also called a plaque-forming unit (PFU).

Realize that the virus infects a host cell, replicates, and spreads to neighboring cells, causing cell death and forming the visible plaque.

Therefore, a single plaque is made up of a localized area of lysed host cells resulting from infection by one infectious virus particle.

주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

Plaque Assay

A plaque assay is a laboratory method used to quantify the number of infectious virus particles in a sample by infecting a layer of host cells. Each infectious virus particle infects a cell and spreads to neighboring cells, creating a clear zone called a plaque where cells have been destroyed.

Plaque Formation

A single plaque forms when one infectious virus particle infects a host cell and replicates, causing cell lysis. The infection spreads locally to adjacent cells, resulting in a visible clear area or plaque, which represents the progeny of that original virus particle.

Relationship Between Plaques and Virus Particles

Each plaque corresponds to one infectious virus particle, also called a plaque-forming unit (PFU). Counting plaques allows estimation of the concentration of infectious viruses in the original sample, assuming each plaque arises from a single virus.