Which of the following accurately describes how a gene for a specific protein gets inserted into a plasmid?

The gene is spliced directly into the plasmid using hydrogen bonds.

Restriction enzymes cut the plasmid and the gene, and DNA ligase seals the gene into the plasmid.

The gene is inserted into the plasmid through ionic interactions between the nucleotide sequences.

RNA polymerase inserts the gene into the plasmid during transcription.