객관식
Which characteristic is shared by the Gram stain, acid-fast stain, and endospore stain?
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- 1. Introduction to Microbiology3h 27m
- Introduction to Microbiology18m
- Introduction to Taxonomy26m
- Scientific Naming of Organisms9m
- Members of the Bacterial World10m
- Introduction to Bacteria9m
- Introduction to Archaea10m
- Introduction to Eukarya20m
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- Bacterial Cell Morphology & Arrangements35m
- Overview of Prokaryotic Cell Structure10m
- Introduction to Bacterial Cell Walls26m
- Gram-Positive Cell Walls11m
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- Introduction to Staining5m
- Simple Staining14m
- Differential Staining6m
- Other Types of Staining11m
- Reviewing the Types of Staining8m
- Gram Stain13m
- 10. Dynamics of Microbial Growth4h 37m
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- 16. Microbial Genetics4h 44m
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- 25. Epidemiology2h 24m
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- Introduction to Chain of Infection5m
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- 26. Applications of the Immune Response2h 9m
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- 33. Microbial Infections - Nervous System2h 18m
- 34. Microbial Infections - Urogenital System1h 40m
9. Microscopes
Gram Stain
객관식
Which of the following lists the correct sequence of reagents used in the Gram stain procedure?
A
Iodine, crystal violet, safranin, alcohol
B
Crystal violet, iodine, alcohol, safranin
C
Alcohol, safranin, crystal violet, iodine
D
Safranin, alcohol, iodine, crystal violet
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검증된 단계별 안내1
Understand that the Gram stain is a differential staining technique used to classify bacteria into Gram-positive and Gram-negative based on their cell wall properties.
Recall the sequence of reagents used in the Gram stain procedure: first, the primary stain crystal violet is applied to stain all cells purple.
Next, iodine is added as a mordant to form a crystal violet-iodine complex, which helps fix the dye inside the cells.
Then, alcohol (usually ethanol or acetone-alcohol) is used as a decolorizer to wash out the dye from Gram-negative cells but not from Gram-positive cells.
Finally, safranin is applied as a counterstain to color the decolorized Gram-negative cells pink/red, allowing differentiation under the microscope.
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