객관식
In the Gram stain procedure, what is the primary purpose of the decolorizer?
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- 1. Introduction to Microbiology3h 27m
- Introduction to Microbiology18m
- Introduction to Taxonomy26m
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- 34. Microbial Infections - Urogenital System1h 40m
9. Microscopes
Gram Stain
객관식
Which of the following correctly lists the order of reagents used in the Gram stain procedure?
A
Safranin, alcohol, iodine, crystal violet
B
Alcohol, crystal violet, safranin, iodine
C
Iodine, crystal violet, safranin, alcohol
D
Crystal violet, iodine, alcohol, safranin
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검증된 단계별 안내1
Understand that the Gram stain procedure is a differential staining technique used to classify bacteria into Gram-positive and Gram-negative based on their cell wall properties.
Recall the sequence of reagents used in the Gram stain: first, the primary stain (crystal violet) is applied to stain all cells purple.
Next, iodine is added as a mordant to form a crystal violet-iodine complex, which helps fix the dye inside the cells.
Then, alcohol (decolorizer) is used to wash away the stain from Gram-negative cells, while Gram-positive cells retain the dye due to their thick peptidoglycan layer.
Finally, safranin is applied as a counterstain to color the decolorized Gram-negative cells pink/red, allowing differentiation under the microscope.
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