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Ch. 7 - The Control of Microbial Growth
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398당신이 사용하는 게 아니라요?교과서 변경
4장, 문제 7

Use the following information to answer questions 6 and 7. The data were obtained from a use-dilution test comparing four disinfectants against Salmonella Choleraesuis.
G = growth, NG = no growth
Table showing bacterial growth (G) or no growth (NG) of Salmonella after exposure to four disinfectants at different dilutions.
Which disinfectant(s) is (are) bactericidal?
a. A,B,C, and D
b. A,C, and D
c. A only
d. B only
e. None of the above

검증된 단계별 안내
1
Understand the difference between bactericidal and bacteriostatic agents: bactericidal disinfectants kill bacteria, resulting in no growth when cultured, while bacteriostatic disinfectants only inhibit growth temporarily.
Review the dilution test data for each disinfectant against Salmonella Choleraesuis, focusing on the presence or absence of bacterial growth (G = growth, NG = no growth) at various concentrations.
Identify which disinfectants show 'no growth' (NG) at the lowest dilution or concentration, indicating that they effectively kill the bacteria rather than just inhibiting growth.
Confirm that the disinfectants classified as bactericidal consistently show no bacterial growth across the tested dilutions, ensuring that the effect is due to killing rather than temporary inhibition.
Based on this analysis, select the disinfectant(s) that meet the criteria for being bactericidal from the given options.

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주요 개념

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Bactericidal vs. Bacteriostatic Agents

Bactericidal agents kill bacteria outright, resulting in no growth when cultured after treatment. In contrast, bacteriostatic agents inhibit bacterial growth but do not kill the bacteria, allowing growth to resume once the agent is removed. Understanding this distinction is key to interpreting disinfectant efficacy.
추천 영상:
04:14
Liquid Chemicals: Surface-Active Agents

Use-Dilution Test Method

The use-dilution test measures the effectiveness of disinfectants by exposing bacteria to different dilutions of the agent and then assessing growth. No growth (NG) indicates effective killing or inhibition at that dilution, while growth (G) indicates survival. This test helps determine whether a disinfectant is bactericidal.
추천 영상:
06:59
The Ames Test

Interpreting Growth Data in Microbial Testing

Interpreting results from microbial tests requires understanding that 'no growth' means the bacteria were killed or inhibited, while 'growth' means survival. For a disinfectant to be considered bactericidal, it must show no growth at the tested concentration, confirming bacterial death rather than just inhibition.
추천 영상:
04:56
Introduction to Controlling Microbial Growth
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