Which of the following cannot be used to sterilize a heat-labile solution stored in a plastic container?
a. Gamma radiation
b. Ethylene oxide
c. Supercritical fluids
d. Autoclaving
e. Short-wavelength radiation

검증된 단계별 안내

Step 1: Understand the term 'heat-labile' which refers to substances that are sensitive to heat and can be denatured or destroyed by high temperatures.

Step 2: Review each sterilization method listed and consider whether it involves high heat that could damage a heat-labile solution or the plastic container.

Step 3: Gamma radiation (a), ethylene oxide (b), supercritical fluids (c), and short-wavelength radiation (e) are sterilization methods that do not rely on high temperatures and are generally safe for heat-sensitive materials.

Step 4: Autoclaving (d) uses high-pressure saturated steam at temperatures around 121°C, which is a high-heat process and can damage heat-labile solutions and plastic containers.

Step 5: Conclude that autoclaving is the sterilization method that cannot be used for heat-labile solutions stored in plastic containers due to its high temperature.

비슷한 문제에 대한 검증된 영상 답변:

이 영상 해법은 위 문제에 도움이 된다고 튜터들이 추천한 것입니다.

영상 길이:

도움이 되었나요?

주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

Heat-labile Solutions

Heat-labile solutions are sensitive to high temperatures and can be damaged or denatured when exposed to heat. Understanding this is crucial because sterilization methods involving heat, such as autoclaving, are unsuitable for these solutions to prevent degradation of their components.

Sterilization Methods and Their Mechanisms

Sterilization methods vary in their approach: autoclaving uses moist heat under pressure, gamma radiation and short-wavelength radiation use ionizing and non-ionizing radiation respectively, ethylene oxide is a chemical sterilant, and supercritical fluids use unique solvent properties. Knowing how each method works helps determine their compatibility with different materials.

Material Compatibility in Sterilization

The container material, such as plastic, affects which sterilization methods can be used without damage. Some methods, like autoclaving, may deform plastics due to heat, while others like ethylene oxide or radiation can sterilize without compromising container integrity. This concept is key to selecting appropriate sterilization techniques.

Which of the following cannot be used to sterilize a heat-labile solution stored in a plastic container?a. Gamma radiationb. Ethylene oxidec. Supercritical fluidsd. Autoclavinge. Short-wavelength radiation