If you put a gene in a virus, the next step in genetic modification would be
a. insertion of a plasmid.
b. transformation.
c. transduction.
d. PCR.
e. Southern blotting.

검증된 단계별 안내

Step 1: Understand the context of the problem, which involves genetic modification using a virus as a vector to carry a gene.

Step 2: Recall that after inserting a gene into a virus, the next step is to introduce this virus into a host cell to deliver the gene.

Step 3: Identify the process by which viruses transfer genetic material into bacterial cells, which is known as transduction.

Step 4: Review the other options: insertion of a plasmid involves plasmids, transformation is uptake of naked DNA, PCR is amplification of DNA, and Southern blotting is a detection method, none of which directly follow gene insertion into a virus.

Step 5: Conclude that the correct next step after putting a gene into a virus is the process of transduction, where the virus transfers the gene into the target cells.

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주요 개념

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Genetic Modification Using Viruses

Viruses can be used as vectors to deliver foreign genes into host cells, a process central to genetic modification. This involves inserting a gene into the viral genome, which then infects the target cell, introducing the new genetic material.

Transduction

Transduction is the process by which viruses transfer genetic material from one bacterium to another. It is a natural mechanism exploited in genetic engineering to introduce new genes into cells using viral vectors.

Transformation vs. Transduction

Transformation involves uptake of naked DNA by cells, while transduction uses viruses to transfer DNA. Understanding the difference helps identify the correct step after inserting a gene into a virus, which is transduction, not transformation.

If you put a gene in a virus, the next step in genetic modification would bea. insertion of a plasmid.b. transformation.c. transduction.d. PCR.e. Southern blotting.