Which of the following is not a functionally analogous pair of stains?
a. Nigrosin and malachite green
b. Crystal violet and carbolfuchsin
c. Safranin and methylene blue
d. Ethanol-acetone and acid-alcohol
e. All of the above pairs are functionally analogous.

검증된 단계별 안내

Step 1: Understand what 'functionally analogous stains' means. These are pairs of stains that serve similar purposes in microbiological staining techniques, such as both being primary stains, counterstains, or decolorizers in different staining protocols.

Step 2: Analyze each pair individually by identifying the role of each stain in common staining methods. For example, nigrosin is a negative stain used for background staining, while malachite green is typically a primary stain in spore staining.

Step 3: Compare the functions of the stains in each pair: determine if both stains are used similarly (e.g., both as primary stains, both as counterstains, or both as decolorizers).

Step 4: For the pair involving ethanol-acetone and acid-alcohol, recognize that both are decolorizing agents but used in different staining protocols (ethanol-acetone in Gram staining and acid-alcohol in acid-fast staining), so they are functionally analogous as decolorizers.

Step 5: Based on the functional roles identified, determine which pair does not share similar staining functions, thus identifying the pair that is not functionally analogous.

비슷한 문제에 대한 검증된 영상 답변:

이 영상 해법은 위 문제에 도움이 된다고 튜터들이 추천한 것입니다.

영상 길이:

도움이 되었나요?

주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

Functionally Analogous Stains

Functionally analogous stains perform similar roles in microbiological staining procedures, such as acting as primary stains, counterstains, or decolorizers. Understanding their function helps differentiate between types of stains and their purpose in highlighting cellular structures.

Types of Stains in Microbiology

Common stains include basic dyes like crystal violet and methylene blue, acidic dyes like nigrosin, and special stains like carbolfuchsin. Each stain has a specific affinity for cellular components, influencing how cells or structures appear under a microscope.

Decolorizing Agents in Staining

Decolorizers such as ethanol-acetone and acid-alcohol remove stains from certain cell types or structures during differential staining. Their role is critical in distinguishing between different bacterial groups, like Gram-positive and Gram-negative bacteria.

Which of the following is not a functionally analogous pair of stains?a. Nigrosin and malachite greenb. Crystal violet and carbolfuchsinc. Safranin and methylene blued. Ethanol-acetone and acid-alcohole. All of the above pairs are functionally analogous.