Which of the following pairs is mismatched?
a. Capsule—negative stain
b. Cell arrangement—simple stain
c. Cell size—negative stain
d. Gram stain—bacterial identification
e. None of the above

검증된 단계별 안내

Step 1: Understand the purpose of each staining technique mentioned in the options. For example, a negative stain is used to visualize capsules and cell size by staining the background, leaving the cells or capsules clear.

Step 2: Analyze option (a): 'capsule—negative stain'. Since negative staining highlights capsules by staining the background and leaving the capsule unstained, this pair is correctly matched.

Step 3: Analyze option (b): 'cell arrangement—simple stain'. Simple stains color the cells uniformly, which helps in observing cell shape and arrangement, so this pair is also correctly matched.

Step 4: Analyze option (c): 'cell size—negative stain'. Negative staining is indeed used to observe cell size because it does not shrink or distort cells, so this pair is correctly matched as well.

Step 5: Analyze option (d): 'Gram stain—bacterial identification'. Gram staining differentiates bacteria into Gram-positive and Gram-negative groups, which is a fundamental step in bacterial identification, so this pair is correctly matched. Therefore, if all pairs are correctly matched, the answer would be (e) none of the above.

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주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

Negative Stain Technique

Negative staining uses acidic dyes that stain the background but not the cells, highlighting structures like capsules and cell size without heat-fixing. It is useful for observing delicate structures and true cell morphology.

Simple Stain and Cell Arrangement

Simple staining employs a single basic dye to color cells, enhancing contrast to observe cell shape and arrangement. It helps identify how bacterial cells group together, such as chains or clusters.

Gram Stain and Bacterial Identification

The Gram stain differentiates bacteria into Gram-positive or Gram-negative based on cell wall properties. This differential staining is fundamental for bacterial classification and guides identification and treatment.

Which of the following pairs is mismatched?a. Capsule—negative stainb. Cell arrangement—simple stainc. Cell size—negative staind. Gram stain—bacterial identificatione. None of the above