design-a-concise-experimental-approach-choose-the-best-combination-to-demonstrat

Question

Design a concise experimental approach (choose the best combination) to demonstrate differential gene expression between cultured muscle and neural cells, indicating the measurement method and expected outcome. Use experimental-design synthesis.

Accepted Answer

Isolate mRNA from each cell type and perform quantitative RT-PCR or RNA sequencing for marker genes; expect high expression of muscle-specific genes in muscle cells and high expression of neuron-specific genes in neural cells.

Answer

Measure total protein concentration per cell and conclude that the type with more total protein is the specialized one, without identifying specific gene products.

Answer

Use light microscopy only to observe cell shape and state that morphological differences alone prove differential gene expression without molecular assays.

Answer

Sequence the entire genome of both cell types and compare DNA sequences expecting many differences that correspond to their functions.