$$TM = \text{ocular lens power} \times \text{objective lens power}$$

Question 1

When transporting a compound microscope, which of the following is the correct method?

Accepted Answer

Hold it by the arm with one hand and support the base with the other hand.

Question 2

Which material should be used to clean the lenses of a compound microscope?

Accepted Answer

Special grit-free lens paper

Question 3

What is the correct formula for calculating the total magnification ($$TM) of a $$specimen viewed under a compound microscope?

Accepted Answer

$$TM = 	ext{ocular lens power} 	imes 	ext{objective lens power}$$

Question 4

If the ocular lens magnifies 10× and the objective lens magnifies 40×, what is the total magnification?

Accepted Answer

$$TM = 10 	imes 40 = 400$$

Question 5

Which step should be performed before storing the microscope?

Accepted Answer

Remove the slide from the stage and rotate the scanning objective lens into position.

Question 6

Why is it necessary to increase light intensity at higher magnifications?

Accepted Answer

The objective lens aperture decreases, allowing less light to enter.

Question 7

What is the resolving power of the compound microscope under ideal conditions?

Accepted Answer

0.2 $$\mum$$

Question 8

Which of the following statements about resolution is correct?

Accepted Answer

Resolution is the ability to discriminate two close objects as separate.

Question 9

A student observes two points that are 0.1 $$\mum $$apart under a compound microscope. What will they see?

Accepted Answer

A single fused image

Question 10

Which adjustment knob should be used with the scanning objective lens?

Accepted Answer

Coarse adjustment knob

Question 11

Why should a coverslip always be used with wet mount preparations?

Accepted Answer

To protect the specimen and prevent contamination of the objective lens.

Question 12

Which of the following actions is NOT recommended when using a compound microscope?

Accepted Answer

Removing parts from the microscope for cleaning.

Question 13

In light microscopy, what is the sequence of image formation?

Accepted Answer

Objective lens forms real image, ocular lens forms virtual image, virtual image is focused on retina.

Question 14

Which factor does NOT affect the resolution of a compound microscope?

Accepted Answer

The color of the specimen

Question 15

A student wants to view a specimen at the highest possible magnification. Which step should they take first?

Accepted Answer

Begin focusing with the scanning objective lens in position.

Question 16

If the objective lens aperture decreases, what is the most likely consequence?

Accepted Answer

Less light enters the objective lens, requiring increased light intensity.

Question 17

Which of the following best describes the relationship between magnification and resolution?

Accepted Answer

Magnification can be increased almost limitlessly, but resolution is limited.

Question 18

A compound microscope has an ocular lens of 15× and an objective lens of 60×. What is the total magnification?

Accepted Answer

$$TM = 15 	imes 60 = 900$$

Question 19

Which of the following is a limitation of the compound light microscope?

Accepted Answer

Objects closer than 0.2 $$\mum $$are seen as a single fused image.

Question 20

In a real-world scenario, why might a pathologist need to increase the light intensity when examining a tissue sample at high magnification?

Accepted Answer

To compensate for the decreased aperture size of the objective lens, which allows less light to enter.

Care, Structure, and Function of the Compound Microscope

Study Guide - Practice Questions