Question 1

Which biosafety level is appropriate for handling Bacillus subtilis, and what is the primary reason for this classification?

Accepted Answer

BSL-1, because it generally does not cause disease in healthy humans.

Question 2

A laboratory technician is working with Staphylococcus aureus. Which biosafety level should be used, and what containment measures are required?

Accepted Answer

BSL-2; containment devices and administrative controls are required.

Question 3

Which organism would require a laboratory to operate at biosafety level 3 due to its potential for causing severe disease?

Accepted Answer

Francisella tularensis

Question 4

A researcher is planning to work with Ebola virus. What biosafety level is required, and what are two key facility requirements for this level?

Accepted Answer

BSL-4; isolated facility and decontamination showers for personnel.

Question 5

Which agency is primarily responsible for regulating occupational safety and health in laboratories in the United States?

Accepted Answer

Occupational Safety and Health Administration (OSHA)

Question 6

A laboratory worker is exposed to a chemical spill. According to the Material Safety Data Sheet (MSDS), what is the first step they should take?

Accepted Answer

Consult the MSDS for first aid measures.

Question 7

Which fire extinguisher type is appropriate for electrical fires, and what symbol is used to identify it?

Accepted Answer

Type C; blue circle

Question 8

A laboratory is using a fume hood to handle volatile chemicals. What is the primary function of the fume hood?

Accepted Answer

To remove hazardous vapors and protect workers from inhalation.

Question 9

Which equation best represents the cumulative effect of repeated radiation exposure, where $$D is $$the total dose and $$d_i is $$the dose from each exposure?

Accepted Answer

$$D = \sum_{i=1}^{n} d_i$$

Question 10

A laboratory worker is required to wear a dosimeter badge. What is the purpose of this badge?

Accepted Answer

To monitor exposure to ionizing radiation.

Question 11

Which organism would be handled in a BSL-2 laboratory but not in a BSL-1 laboratory?

Accepted Answer

Streptococcus pyogenes

Question 12

What is the primary reason for using an autoclave in a microbiological laboratory?

Accepted Answer

To sterilize equipment and kill microorganisms using high-pressure steam.

Question 13

Which equation describes the relationship between pressure ($$P$$), volume ($$V$$), and temperature ($$T) in an $$autoclave, assuming ideal gas behavior?

Accepted Answer

$$PV = nRT$$

Question 14

A laboratory worker accidentally splashes a corrosive chemical into their eyes. What is the immediate action they should take?

Accepted Answer

Flush their eyes at the eyewash station for at least 15 minutes.

Question 15

Which type of waste should be disposed of in a sharps container labeled with a biohazard symbol?

Accepted Answer

Used syringes and needles contaminated with infectious material.

Question 16

Which personal protective equipment (PPE) is mandatory when working with hazardous chemicals that pose a risk to the eyes?

Accepted Answer

Safety goggles or face shield

Question 17

A hospital must comply with OSHA regulations for hazard communication and training. Which of the following is a key requirement?

Accepted Answer

Providing MSDSs for all chemicals present in the facility.

Question 18

Which biosafety level requires personnel to take decontamination showers each time they leave the laboratory?

Accepted Answer

BSL-4

Question 19

A laboratory is handling Yellow fever virus. What biosafety level is required, and what is a key safety feature of this level?

Accepted Answer

BSL-3; work must be done in biological safety cabinets with sealed windows.

Question 20

Which of the following is a common misconception about laboratory gloves?

Accepted Answer

Gloves are a substitute for proper hand washing.

Chapter 9

Study Guide - Practice Questions

Study Guide - Flashcards