Q: What is the correct formula for calculating the total magnification ($$TM) of a $$specimen viewed under a compound microscope?

$$TM = \text{ocular lens power} \times \text{objective lens power}$$

Question 1

When transporting a compound microscope, which of the following is the correct procedure?

Accepted Answer

Hold it in an upright position with one hand on the arm and the other supporting the base.

Question 2

Which material should be used to clean the lenses of a compound microscope?

Accepted Answer

Special grit-free lens paper.

Question 3

What is the correct formula for calculating the total magnification ($$TM) of a $$specimen viewed under a compound microscope?

Accepted Answer

$$TM = 	ext{ocular lens power} 	imes 	ext{objective lens power}$$

Question 4

If the ocular lens magnifies 10× and the objective lens magnifies 40×, what is the total magnification?

Accepted Answer

$$10 	imes 40 = 400$$

Question 5

Which step should be performed before storing the microscope?

Accepted Answer

Remove the slide, rotate the scanning objective lens into position, wrap the cord, and cover the microscope.

Question 6

Why is it necessary to increase light intensity at higher magnifications?

Accepted Answer

Because the objective lens aperture decreases, allowing less light in.

Question 7

What is the resolving power of the compound microscope under ideal conditions?

Accepted Answer

0.2 μm

Question 8

Which lens should be used with the coarse adjustment knob?

Accepted Answer

Scanning objective lens

Question 9

What is the primary function of the coverslip in wet mount preparations?

Accepted Answer

To protect the specimen and prevent contamination.

Question 10

If two objects are 0.1 μm apart, how will they appear under a compound microscope?

Accepted Answer

As a single fused image.

Question 11

Which of the following best describes the process of image formation in a compound microscope?

Accepted Answer

The objective lens forms the real image, and the ocular lens forms the virtual image.

Question 12

A student is using a compound microscope and notices that the image is not clear at high magnification. Which adjustment should they make first?

Accepted Answer

Increase the light intensity.

Question 13

Which of the following actions is NOT recommended when using a compound microscope?

Accepted Answer

Removing parts from the microscope if there is a mechanical problem.

Question 14

If the human eye can resolve objects about 100 μm apart, but the compound microscope can resolve objects 0.2 μm apart, what is the improvement factor in resolution?

Accepted Answer

$$\frac{100}{0.2} = 500$$

Question 15

A student uses a 10× ocular lens and a 100× oil immersion objective lens. What is the total magnification?

Accepted Answer

$$10 	imes 100 = 1000$$

Question 16

Which factor most directly affects the resolution of a compound microscope?

Accepted Answer

The amount and physical properties of visible light entering the microscope.

Question 17

Why should you never remove any parts from the microscope if a mechanical problem arises?

Accepted Answer

Because it can damage the precision instrument and should be handled by an instructor.

Question 18

In the diagram provided, what is the role of the light source in the compound microscope?

Accepted Answer

To illuminate the specimen for better resolution and image formation.

Question 19

A student prepares a wet mount without a coverslip. What is the most likely consequence?

Accepted Answer

The specimen may dry out or become contaminated.

Question 20

Which of the following best explains why the compound microscope is considered a precision instrument?

Accepted Answer

It requires careful handling and maintenance to ensure accurate magnification and resolution.

Microscopy: Structure, Care, and Principles of the Compound Microscope

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