Q: If the field of view at 4X is 4.5 mm, what is the mathematical relationship to calculate the field of view at a higher magnification?

$$\text{FOV}_{\text{new}} = \frac{\text{FOV}_{\text{old}} \times \text{Magnification}_{\text{old}}}{\text{Magnification}_{\text{new}}}$$

Question 1

Which part of the microscope is responsible for controlling the amount of light that passes through the specimen?

Accepted Answer

Iris diaphragm lever

Question 2

If the field of view (FOV) with the 4X objective lens is 4.5 mm, what is the FOV when using the 40X objective lens?

Accepted Answer

0.45 mm

Question 3

What is the total magnification when using a 40X objective lens with a 10X ocular lens?

Accepted Answer

$$40 	imes 10 = 400$$

Question 4

Which of the following best describes the term 'parfocal' in microscopy?

Accepted Answer

The ability to maintain focus when switching between objective lenses

Question 5

A student observes a specimen under the 10X objective lens and estimates the field of view to be 1.8 mm. If a cell spans approximately one-fourth of the field, what is the estimated size of the cell?

Accepted Answer

$$1.8 \div 4 = 0.45 mm$$

Question 6

Which adjustment knob should you use first when focusing on a specimen with the 4X objective lens?

Accepted Answer

Coarse adjustment knob

Question 7

When you move a slide to the right on the stage, in which direction does the image appear to move when viewed through the microscope?

Accepted Answer

To the left

Question 8

Which of the following best defines 'resolution' in microscopy?

Accepted Answer

The ability to distinguish two points as separate

Question 9

If you switch from a 10X to a 100X objective lens, what happens to the field of view?

Accepted Answer

It decreases

Question 10

A student is using the 40X objective lens and the field of view is 0.45 mm. If a cell takes up half the field, what is the approximate size of the cell?

Accepted Answer

$$0.45 \div 2 = 0.225 mm$$

Question 11

Which part of the microscope should you adjust to increase the contrast of your specimen?

Accepted Answer

Iris diaphragm lever

Question 12

What is the main function of the condenser in a microscope?

Accepted Answer

To focus light onto the specimen

Question 13

Which of the following statements about working distance is correct?

Accepted Answer

Working distance decreases as magnification increases

Question 14

If the field of view at 4X is 4.5 mm, what is the mathematical relationship to calculate the field of view at a higher magnification?

Accepted Answer

$$	ext{FOV}_{	ext{new}} = \frac{	ext{FOV}_{	ext{old}} 	imes 	ext{Magnification}_{	ext{old}}}{	ext{Magnification}_{	ext{new}}}$$

Question 15

Which of the following best describes 'depth of field' in microscopy?

Accepted Answer

The vertical range in which the specimen remains in focus

Question 16

A student is viewing a wet mount of cheek cells under the microscope. Which technique should be used to avoid breaking the slide when switching to a higher power objective?

Accepted Answer

Use only the fine adjustment knob

Question 17

When using a microscope, why is it important to start focusing with the lowest power objective lens?

Accepted Answer

It provides the largest field of view and reduces the risk of damaging the slide

Question 18

Which part of the microscope holds the objective lenses and allows you to switch between them?

Accepted Answer

Revolving nosepiece

Question 19

If you are viewing a specimen at 100X total magnification and switch to 400X, what happens to the field of view?

Accepted Answer

It becomes four times smaller

Question 20

Which of the following is NOT a function of the stage motion knobs?

Accepted Answer

Adjusting the focus

Microscopy: Structure, Function, and Application in Anatomy & Physiology

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