Given a mixture of proteins, how would you expect the elution order to change if the pore size of the beads is increased?

• A. Smaller proteins would elute first, followed by larger proteins.
• B. All proteins would elute at the same time.
• C. Larger proteins would still elute first, but smaller proteins would elute faster than before.
• D. The elution order would remain unchanged.

Answer: (C)