A protein with 100 amino acids is sequenced using Edman degradation. Explain how the accumulation of unwanted PTH amino acid side products affects the sequencing accuracy.

• A. The side products are converted back to the original amino acids, maintaining accuracy.
• B. The side products enhance the reaction efficiency, improving sequencing accuracy.
• C. The side products obscure the results, making it difficult to identify the correct sequence.
• D. The side products are easily separated from the desired products, having no effect on accuracy.

Answer: (C)