Why is it challenging to determine the order of peptide fragments in the original protein sequence after cleavage?

• A. The original sequence is lost during the cleavage process.
• B. Cleavage reagents often modify the amino acids, altering the sequence.
• C. Cleavage can produce fragments of similar size, making it difficult to determine their order.
• D. Fragments are often degraded before sequencing can occur.

Answer: (C)