What is the primary difference between anion-exchange chromatography and cation-exchange chromatography?

In anion-exchange chromatography, how are negatively charged proteins separated from a mixture?

How does the net charge of proteins influence their elution order in anion-exchange chromatography?

Which type of protein elutes first in anion-exchange chromatography?

In a mixture containing proteins with net charges of +3, 0, and -5, which protein would elute last in an anion-exchange chromatography column?

What is the function of DEAE resin in anion-exchange chromatography?

What is the role of salt in the elution of negatively charged proteins during anion-exchange chromatography?