In anion-exchange chromatography, how are negatively charged proteins separated from a mixture?

• A. Negatively charged proteins bind to neutral resin and are eluted second.
• B. Negatively charged proteins pass through the column without binding and are eluted first.
• C. Negatively charged proteins bind to the positively charged resin and are eluted last.
• D. Negatively charged proteins bind to negatively charged resin and are eluted first.

Answer: (C)