How does the net charge of proteins influence their elution order in anion-exchange chromatography?

• A. Proteins with greater net positive charge elute first, while those with greater net negative charge elute last.
• B. Proteins with greater net charge elute first, regardless of being positive or negative.
• C. Proteins with neutral charge elute first, while those with any charge elute last.
• D. Proteins with greater net negative charge elute first, while those with greater net positive charge elute last.

Answer: (A)