How would a non-competitive inhibitor with alpha = 1 and alpha prime = 2 affect the apparent Km and Vmax of an enzyme with a Km of 15 mM and Vmax of 250 μmol/min?

• A. Apparent Km increases, and apparent Vmax remains unchanged.
• B. Apparent Km increases, and apparent Vmax decreases.
• C. Apparent Km remains unchanged, and apparent Vmax decreases.
• D. Apparent Km decreases, and apparent Vmax increases.

Answer: (C)