Given the inhibition constants KI = 0.1 M and K'I = 0.5 M, which complex has a higher affinity for the inhibitor?

• A. Affinity cannot be determined from inhibition constants.
• B. The free enzyme has a higher affinity for the inhibitor.
• C. The enzyme-substrate complex has a higher affinity for the inhibitor.
• D. Both complexes have equal affinity for the inhibitor.

Answer: (B)