How can the inhibition constant for the free enzyme (KI) and the enzyme-substrate complex (K'I) be used to determine the preferred binding site of an inhibitor?

• A. By comparing KI and K'I values, the site with the lower inhibition constant indicates the preferred binding site.
• B. By comparing KI and K'I values, the site with the higher inhibition constant indicates the preferred binding site.
• C. The preferred binding site is determined by the substrate concentration, not KI or K'I values.
• D. The preferred binding site is determined by the enzyme's reaction velocity, not KI or K'I values.

Answer: (A)