Design an experiment to test the role of the hydrophobic effect in protein folding.

• A. Measure the rate of protein synthesis in a cell-free system.
• B. Compare the solubility of proteins in different pH environments.
• C. Analyze the effect of temperature on enzyme activity.
• D. Use a denaturant to unfold a protein and observe refolding in the presence and absence of hydrophobic interactions.

Answer: (D)