A mutation prevents DAG from remaining associated with the plasma membrane. Predict the impact on PKC activation.

• A. PKC would activate other secondary messengers instead.
• B. PKC would be unaffected as it does not require DAG for activation.
• C. PKC activation would be impaired, reducing phosphorylation of target proteins.
• D. PKC activation would increase, enhancing phosphorylation of target proteins.

Answer: (C)