Skip to main content
Back

2D-Electrophoresis definitions

Control buttons has been changed to "navigation" mode.
1/15
  • 2D-Electrophoresis
    Technique combining two separation methods to resolve proteins by both isoelectric point and molecular weight in perpendicular directions.
  • Isoelectric Focusing
    Method using a stable pH gradient to concentrate proteins at their unique isoelectric point, enabling separation by charge.
  • SDS-PAGE
    Gel-based process separating proteins by molecular weight, with heavier proteins migrating slower and appearing higher in the gel.
  • pH Gradient
    Continuous range of pH values within a gel, crucial for focusing proteins at their isoelectric points during separation.
  • Isoelectric Point
    Specific pH where a protein carries no net charge, causing it to stop migrating in an electric field.
  • Molecular Weight
    Physical property determining how fast a protein moves through a gel, with smaller proteins traveling further.
  • Protein Band
    Visual representation of proteins in a gel, indicating their position based on separation criteria like pI or molecular weight.
  • Proteomics
    Field focused on large-scale study and analysis of proteins, often using advanced separation techniques for characterization.
  • Charge–Charge Interaction
    Electrostatic relationship influencing protein migration during isoelectric focusing, based on their net charge at different pH.
  • Amino Acid Analysis
    Process of examining protein composition, facilitated by separation techniques that resolve individual proteins.
  • Electric Field
    Force applied to gels, driving protein migration according to their charge or size during electrophoresis.
  • Gel Matrix
    Supportive medium in electrophoresis, providing structure for protein separation by pI or molecular weight.
  • Protein Spot
    Distinct area in a 2D gel corresponding to a unique protein, separated by both isoelectric point and molecular weight.
  • Axis Label
    Indicator on a gel image specifying which dimension represents isoelectric point or molecular weight, essential for interpretation.
  • Protein Characterization
    Comprehensive analysis of protein properties, enabled by resolving proteins through advanced separation techniques.