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Calculating Km definitions

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  • Michaelis constant
    Represents the substrate concentration at which the initial reaction velocity is half of the maximum velocity in enzyme kinetics.
  • Michaelis-Menten equation
    Describes the relationship between reaction velocity, substrate concentration, and enzyme parameters in enzyme-catalyzed reactions.
  • Lineweaver-Burk equation
    A reciprocal form of the Michaelis-Menten equation used to linearize enzyme kinetic data for easier parameter determination.
  • Substrate concentration
    Amount of reactant present in an enzyme-catalyzed reaction, crucial for determining reaction velocity and Km.
  • Initial reaction velocity
    The rate of product formation at the very start of an enzyme-catalyzed reaction, before substrate depletion.
  • Maximum velocity
    The highest possible rate of an enzyme-catalyzed reaction when substrate is abundant and enzyme is saturated.
  • Enzyme-substrate complex
    A temporary molecular assembly formed when an enzyme binds its substrate, central to kinetic calculations.
  • Dissociation rate constant
    A parameter indicating how quickly the enzyme-substrate complex breaks apart into free enzyme and substrate or product.
  • Association rate constant
    A parameter reflecting how rapidly free enzyme and substrate combine to form the enzyme-substrate complex.
  • Affinity
    A measure of how strongly an enzyme binds its substrate, with higher values indicated by lower Km.
  • Free enzyme
    Unbound enzyme available to interact with substrate, its concentration influences kinetic calculations.
  • Free substrate
    Unbound reactant available for enzyme interaction, its concentration is used in kinetic equations.
  • Rate constant
    A numerical value describing the speed of association or dissociation events in enzyme kinetics.
  • Reciprocal plot
    A graphical method using inverse values to linearize kinetic data, facilitating parameter extraction.
  • Enzyme catalysis
    The process by which enzymes accelerate chemical reactions, forming and breaking enzyme-substrate complexes.