Skip to main content
Back

Protein Extraction definitions

Control buttons has been changed to "navigation" mode.
1/15
  • Protein Extraction
    Initial process for obtaining proteins from cells, resulting in a mixture of cellular components rather than purified proteins.
  • Cell Homogenization
    Procedure that breaks open cells to release their internal contents into a solution, forming a crude extract.
  • Crude Extract
    Mixture containing all released cellular contents, including organelles and molecules, after cell disruption.
  • Homogenate
    Collective term for the total contents released from cells following homogenization.
  • Organelles
    Cellular structures such as nucleus, endoplasmic reticulum, and mitochondria present in the crude extract.
  • Nucleus
    Membrane-bound organelle containing genetic material, found among the components in a crude extract.
  • Endoplasmic Reticulum
    Organelle involved in protein and lipid synthesis, released during cell homogenization.
  • Mitochondria
    Energy-producing organelles found in the mixture after cell disruption.
  • Lysis Buffers
    Solutions used to interact with cell membranes and facilitate cell breakage during homogenization.
  • Blender
    Instrument used to physically disrupt cells, aiding in the release of cellular contents.
  • Homogenizer
    Device designed to mechanically break open cells for extraction of internal components.
  • Sonication
    Technique employing sound waves to disrupt cell membranes and release cellular contents.
  • Red Blood Cell
    Example cell used in protein extraction, providing a source of proteins and other cellular structures.
  • Differential Centrifugation
    Subsequent step after extraction, used to further separate and purify proteins from the crude mixture.
  • Plasma Membrane
    Cell boundary targeted by lysis buffers to facilitate cell disruption during extraction.