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A Tour of the Cell: Structure and Function in Eukaryotic and Prokaryotic Cells

Study Guide - Smart Notes

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Introduction to the Cell

Microscopes Reveal the World of the Cell

Microscopes are essential tools for studying cell structure and function. The light microscope allows observation of living cells, while electron microscopes (scanning and transmission) provide greater magnification and resolution, revealing cellular ultrastructure. Magnification is the increase in an object's image size compared to its actual size, and resolution is the clarity of an image, or the ability to distinguish two nearby objects as separate.

  • Cell theory: All living things are composed of cells, and all cells come from other cells.

  • Microscopy has enabled the discovery and understanding of cell structure and function.

Light micrograph of Paramecium Scanning electron micrograph of Paramecium Transmission electron micrograph of Toxoplasma Differential interference contrast micrograph of Paramecium Microscope scale and cell size comparison

Cell Size and the Plasma Membrane

The Small Size of Cells Relates to the Need to Exchange Materials

Most cells are microscopic, which provides a large surface-to-volume ratio. This ratio is crucial for efficient exchange of materials across the plasma membrane. The plasma membrane is a phospholipid bilayer with embedded proteins, some forming channels for ions and hydrophilic molecules, others acting as pumps for active transport.

  • Surface-to-volume ratio limits cell size; smaller cells have more surface area relative to their volume.

  • Membrane proteins facilitate selective transport and communication.

Surface-to-volume ratio comparison Structure of the plasma membrane

Prokaryotic and Eukaryotic Cells

Prokaryotic Cells Are Structurally Simpler

All cells share basic features: plasma membrane, DNA, ribosomes, and cytosol. Prokaryotic cells (Bacteria and Archaea) are smaller and simpler, lacking a membrane-bound nucleus and organelles. Their DNA is located in the nucleoid region, and they may have flagella for movement.

  • Prokaryotic cells: No nucleus, no membrane-bound organelles.

  • Eukaryotic cells: Membrane-enclosed nucleus and organelles.

Structure of a prokaryotic cell

Eukaryotic Cells Are Partitioned into Functional Compartments

Eukaryotic cells (domain Eukarya) are characterized by compartmentalization via membrane-enclosed organelles. These organelles perform specialized functions and are organized into four functional groups:

  1. Genetic control: Nucleus and ribosomes

  2. Manufacture, distribution, breakdown: Endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, peroxisomes

  3. Energy processing: Mitochondria (all cells), chloroplasts (plant cells)

  4. Structural support, movement, communication: Cytoskeleton, plasma membrane, cell wall (plants)

Animal cell structure Plant cell structure

The Nucleus and Ribosomes

The Nucleus Contains the Cell’s Genetic Instructions

The nucleus houses DNA, which directs protein synthesis via messenger RNA (mRNA). The nucleolus is the site of ribosome subunit assembly.

Structure of the nucleus

Ribosomes Make Proteins for Use in the Cell and Export

Ribosomes are composed of ribosomal RNA (rRNA) and proteins. They synthesize proteins according to instructions from DNA. Cells with high protein production have many ribosomes.

Ribosome structure and function

The Endomembrane System

Many Organelles Are Connected in the Endomembrane System

The endomembrane system includes organelles that interact in the synthesis, distribution, storage, and export of molecules. These organelles are structurally and functionally interconnected.

The Endoplasmic Reticulum (ER) is a Biosynthetic Workshop

The ER is a network of tubes and sacs. Smooth ER synthesizes lipids and processes toxins. Rough ER produces membranes, and its ribosomes make membrane and secretory proteins.

Structure of the endoplasmic reticulum Synthesis and packaging of a secretory protein by the rough ER

The Golgi Apparatus Modifies, Sorts, and Ships Cell Products

The Golgi apparatus consists of stacks of sacs where products from the ER are processed and sent to other organelles or the cell surface.

Structure and function of the Golgi apparatus

Lysosomes Are Digestive Compartments Within a Cell

Lysosomes contain enzymes that break down ingested substances and damaged organelles, facilitating recycling within the cell.

Lysosome digesting food vacuole Lysosome digesting damaged organelle

Vacuoles Function in the General Maintenance of the Cell

Vacuoles are large vesicles with diverse functions. Protists have contractile vacuoles for expelling excess water, while plant cells have a central vacuole for storage and growth.

Contractile vacuole in Paramecium

Energy-Converting Organelles

Mitochondria Harvest Chemical Energy from Food

Mitochondria are organelles that carry out cellular respiration in eukaryotic cells. They have two internal compartments: the intermembrane space and the mitochondrial matrix, which contains DNA, ribosomes, and enzymes for respiration.

Chloroplasts Convert Solar Energy to Chemical Energy

Chloroplasts are the photosynthesizing organelles of plants and algae, converting light energy into the chemical energy of sugars.

Evolution Connection: Mitochondria and Chloroplasts Evolved by Endosymbiosis

The endosymbiont theory proposes that mitochondria and chloroplasts were once small prokaryotes that began living within larger cells, explaining their similarities to prokaryotes.

The Cytoskeleton and Cell Surfaces

The Cell’s Internal Skeleton Helps Organize Its Structure and Activities

The cytoskeleton is composed of microfilaments, intermediate filaments, and microtubules. These fibers maintain cell shape, anchor and move organelles, enable amoeboid movement, and facilitate muscle contraction.

Cilia and Flagella Move When Microtubules Bend

Eukaryotic cilia and flagella are locomotor appendages made of microtubules in a "9 + 2" arrangement. Flagella propel cells with a whiplike motion, while cilia move in a coordinated, oar-like fashion.

The Extracellular Matrix of Animal Cells Functions in Support and Regulation

Animal cells synthesize and secrete an extracellular matrix (ECM) that binds tissue cells, supports the plasma membrane, and communicates with the cytoskeleton. The ECM attaches to cells via glycoproteins and integrins.

Three Types of Cell Junctions Are Found in Animal Tissues

Specialized junctions allow cells to adhere, interact, and communicate:

  • Tight junctions: Bind cells to form leakproof sheets.

  • Anchoring junctions: Rivet cells into strong tissues.

  • Gap junctions: Allow ions and small molecules to flow between cells.

Cell Walls Enclose and Support Plant Cells

Plant cells have a rigid cell wall composed primarily of cellulose, providing protection and structural support. Plasmodesmata are cell junctions that allow plant tissues to share water, nutrients, and chemical messages.

Review: Eukaryotic Cell Structures and Their Functions

Functional Categories of Organelles

Eukaryotic cell structures can be grouped by function:

  1. Genetic control

  2. Manufacturing, distribution, breakdown

  3. Energy processing

  4. Structural support, movement, communication

Table: Eukaryotic Cell Structures and Their Functions

Organelle

Main Function

Nucleus

Genetic control

Ribosomes

Protein synthesis

Endoplasmic Reticulum

Synthesis and processing of proteins/lipids

Golgi Apparatus

Modification, sorting, shipping of cell products

Lysosomes

Digestion and recycling

Vacuoles

Storage and maintenance

Mitochondria

Energy conversion (cellular respiration)

Chloroplasts

Energy conversion (photosynthesis)

Cytoskeleton

Structural support, movement

Plasma Membrane

Selective transport, communication

Cell Wall (plants)

Protection, support

Metric Measurement Equivalents

Understanding cell size requires knowledge of metric units:

  • 1 meter (m) = 100 centimeters (cm) = 1,000 millimeters (mm)

  • 1 centimeter (cm) = 0.01 m

  • 1 millimeter (mm) = 0.001 m

  • 1 micrometer (μm) = 0.000001 m

  • 1 nanometer (nm) = 0.000000001 m

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