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DNA, Gene Expression, Mutations, Gene Regulation, and Biotechnology: Study Guide

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The Griffith Experiment

  • The Griffith Experiment
    08:56

The Hershey-Chase Experiment

  • The Hershey-Chase Experiment
    03:59
  • Bacteriophages
    03:44

Chargaff's Rules

  • Chargaff's Rules
    04:33

Discovering the Structure of DNA

  • Detailed DNA Structure
    06:24
  • Discovering the Structure of DNA
    04:41

Introduction to DNA Replication

  • Unwinding the DNA: Topoisomerase, Helicase & SSBs
    05:10
  • Components of DNA Replication
    04:47
  • Origin of Replication
    03:33

DNA Polymerases

  • DNA Polymerase Requirements
    05:03
  • DNA Polymerases
    04:01

Leading & Lagging DNA Strands

  • Leading & Lagging DNA Strands
    05:15

DNA Repair

  • DNA Repair
    03:20
  • DNA Proofreading & Repair Enzymes
    02:12

Central Dogma

  • Central Dogma
    05:45

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • 3) Termination of Transcription
    02:44
  • 1) Initiation of Transcription
    04:16
  • 2) Elongation of Transcription
    04:01

Eukaryotic RNA Processing and Splicing

  • Eukaryotic RNA Processing and Splicing
    02:32
  • 2) RNA Splicing Creates Mature mRNA
    08:44
  • 1) RNA Processing
    04:19

Introduction to Types of RNA

  • Introduction to Types of RNA
    07:20

Genetic Code

  • How to Use the Genetic Code
    11:39
  • Genetic Code
    01:28

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Steps of Translation

  • 2) Elongation of Translation
    04:59
  • 3) Termination of Translation
    02:16
  • 1) Initiation of Translation
    05:03

Mutations

  • Mutations
    02:29
  • Types of Mutations
    08:44

Introduction to Regulation of Gene Expression

  • Positive vs Negative Gene Regulation
    02:17
  • Introduction to Regulation of Gene Expression
    06:40

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

The Trp Operon

  • In the Presence of Tryptophan
    03:11
  • The Trp Operon
    05:30
  • In the Absence of Tryptophan
    03:42

Review of the Lac Operon & Trp Operon

  • Review of the Lac Operon & Trp Operon
    06:24

Introduction to Eukaryotic Gene Regulation

  • Map of Eukaryotic Gene Regulation
    04:22
  • Introduction to Eukaryotic Gene Regulation
    03:47

Eukaryotic Chromatin Modifications

  • Eukaryotic Chromatin Modifications
    03:55
  • Histone Acetylation
    04:14
  • DNA Methylation
    03:39

Eukaryotic Transcriptional Control

  • Introduction to Transcription Factors
    03:32
  • General Transcription Factors
    03:51
  • Specific Transcription Factors
    03:57

Eukaryotic Post-Transcriptional Regulation

  • Eukaryotic Post-Transcriptional Regulation
    01:56
  • 3) RNA Interference
    04:16
  • 1) Alternative RNA Splicing
    05:17

Eukaryotic Post-Translational Regulation

  • Protein Ubiquitination
    02:45
  • Eukaryotic Post-Translational Regulation
    03:55

Gel Electrophoresis

  • Gel Electrophoresis
    07:58
  • Gel Electrophoresis Example 1
    02:34

Introduction to Polymerase Chain Reaction

  • Introduction to Polymerase Chain Reaction
    01:31
  • Components of a Polymerase Chain Reaction
    05:57
  • Why do we use PCR?
    02:53

The Steps of PCR

  • 1) Denaturation
    03:33
  • 2) Annealing
    02:56
  • The Steps of PCR
    04:39

Southern Blotting

  • Southern Blotting
    03:31
  • Steps of Southern Blotting
    08:21

Introduction to DNA Cloning

  • Introduction to DNA Cloning
    02:25
  • Cloning with Recombinant DNA
    03:41

Steps to DNA Cloning

  • Steps to DNA Cloning
    04:42
  • 1b) Use Ligation Enzymes
    04:58
  • 1a) Use Restriction Enzymes
    03:27

Genomes

  • Gene Families
    02:06
  • Transposable Elements and Pseudogenes
    03:57
  • Protein Encoding Genes
    02:11

Dideoxy Sequencing

  • Chain-Termination PCR
    07:37
  • Dideoxy Sequencing
    01:03
  • Determining the DNA Sequence from a Gel
    06:28