Q: Which formula is used to calculate the field of view (FOV) in microscopy?

$$\text{FOV} = \frac{\text{Field number}}{\text{Total magnification}}$$

Q: A student is using a microscope with a 10x ocular lens and a 4x objective lens. The field number of the ocular lens is 18 mm. What is the field of view at this magnification?

$$\text{FOV} = \frac{18}{10 \times 4} = \frac{18}{40} = 0.45 mm$$

Question 1

Which of the following statements correctly describes the difference between optical and electron microscopes?

Accepted Answer

Optical microscopes use visible light and lenses to magnify specimens, while electron microscopes use beams of electrons for illumination.

Question 2

A compound microscope has an ocular lens with a magnification of 10x and an objective lens with a magnification of 40x. What is the total magnification of the specimen?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

Which formula is used to calculate the field of view (FOV) in microscopy?

Accepted Answer

$$	ext{FOV} = \frac{	ext{Field number}}{	ext{Total magnification}}$$

Question 4

Why are specimens best viewed when thinly sectioned or flattened under a compound microscope?

Accepted Answer

Because compound microscopes have a shallow depth of field, allowing only thin layers to be in focus.

Question 5

Which of the following is a correct statement about wet mounts?

Accepted Answer

Wet mounts permit immediate viewing of specimens and do not require curing or drying periods.

Question 6

Lugol's iodine (IKI) is commonly used in microscopy for which purpose?

Accepted Answer

To turn starches black for easier visualization.

Question 7

Which of the following functional groups is correctly matched with its chemical formula?

Accepted Answer

Phosphate group: $$-PO_4$

Question 8

Which class of biological molecules is NOT a polymer?

Accepted Answer

Lipids

Question 9

A student observes a specimen under a microscope using a 15x ocular lens and a 100x oil immersion objective lens. What is the total magnification?

Accepted Answer

$$15 	imes 100 = 1500$$

Question 10

Which of the following best explains why field of view decreases as magnification increases?

Accepted Answer

Because the total area visible through the microscope is inversely proportional to magnification.

Question 11

Which functional group is found in all amino acids and is essential for protein structure?

Accepted Answer

Amino group ($$-NH_2$)

Question 12

Which type of electron microscope is best suited for viewing the internal structure of cells?

Accepted Answer

Transmission electron microscope (TEM)

Question 13

A scientist wants to estimate the size of a cell using a microscope. The field of view is 0.5 mm and the cell occupies one-fourth of the field. What is the approximate size of the cell?

Accepted Answer

$$0.5 \div 4 = 0.125 mm$$

Question 14

Which of the following is a correct statement about the depth of field in compound microscopes?

Accepted Answer

Depth of field decreases as magnification increases.

Question 15

Which of the following biological molecules is primarily responsible for storing genetic information?

Accepted Answer

Nucleic acids

Question 16

Which functional group is responsible for the acidic properties of organic acids?

Accepted Answer

Carboxyl group ($$-COOH)$$

Question 17

Which of the following statements about polymers is correct?

Accepted Answer

Proteins are polymers made of amino acid monomers.

Question 18

A student is using a microscope with a 10x ocular lens and a 4x objective lens. The field number of the ocular lens is 18 mm. What is the field of view at this magnification?

Accepted Answer

$$	ext{FOV} = \frac{18}{10 	imes 4} = \frac{18}{40} = 0.45 mm$$

Question 19

Which of the following is NOT a function of proteins in biological systems?

Accepted Answer

Storing genetic information

Microscopy and Biological Macromolecules: Study Notes

Study Guide - Practice Questions

Study Guide - Flashcards