Question 1

Which part of the compound microscope is responsible for supporting the slide and allowing light to pass through the specimen?

Accepted Answer

Stage

Question 2

A student observes a specimen using a compound microscope with an ocular lens of $$10	imes$$ and an objective lens of $$40	imes. $$What is the total linear magnification?

Accepted Answer

$$400	imes$$

Question 3

Which of the following is the correct formula for calculating the magnification of a drawing?

Accepted Answer

$$M = \dfrac{SD}{SO}$$

Question 4

When using the high power objective (HPO), which precaution should you take to avoid damaging the microscope?

Accepted Answer

Only use the fine adjustment knob for focusing

Question 5

A student draws a specimen that is $$80\,\mathrm{mm}$$ long, while the actual specimen is $$8\,\mathrm{mm}$$ long. What is the magnification of the drawing?

Accepted Answer

$$M = \dfrac{80}{8} = 10$$

Question 6

Which optical part of the microscope is used to concentrate light onto the specimen?

Accepted Answer

Condenser

Question 7

If the objective lens is $$10	imes$$ and the ocular lens is $$15	imes$$, what is the total magnification?

Accepted Answer

$$150	imes$$

Question 8

Why is it important to use only lens tissue when cleaning microscope lenses?

Accepted Answer

Other materials may scratch or damage the lens surface

Question 9

Which part of the microscope allows you to change the objective lenses to increase or decrease magnification?

Accepted Answer

Revolving nosepiece

Question 10

A student prepares a temporary mount of a specimen. Which of the following is NOT a step in preparing a temporary mount?

Accepted Answer

Fixing the specimen in fluid

Question 11

If a microscope slide is dirty, which part should be cleaned first before viewing?

Accepted Answer

The ocular lens

Question 12

Which of the following best describes the function of the diaphragm in a compound microscope?

Accepted Answer

It adjusts the amount of light passing through the specimen

Question 13

A student is using a compound microscope and wants to observe a specimen at the lowest magnification. Which objective lens should they use?

Accepted Answer

Scanner

Question 14

Which of the following is a correct step when focusing a compound microscope?

Accepted Answer

Start with the low power objective and use coarse adjustment

Question 15

A student draws a skin hair at high power objective (HPO) and measures the drawing as $$50\,\mathrm{mm}$$ long. The actual hair is $$0.5\,\mathrm{mm}$$ long. What is the magnification of the drawing?

Accepted Answer

$$M = \dfrac{50}{0.5} = 100$$

Question 16

Which of the following is a reason for using permanent mounts in microscopy?

Accepted Answer

To preserve specimens for long-term study

Question 17

If the actual length of a specimen is $$12\,\mathrm{mm}$$ and the drawing is $$96\,\mathrm{mm}$$, what is the magnification?

Accepted Answer

$$M = \dfrac{96}{12} = 8$$

Question 18

Which part of the microscope should be rotated to switch between different objective lenses?

Accepted Answer

Revolving nosepiece

Question 19

Why is it important to avoid using the coarse adjustment knob when the high power objective is in place?

Accepted Answer

It can break the slide or damage the lens due to close proximity

Question 20

A student wants to observe the fine details of a specimen. Which objective lens should they use?

Accepted Answer

High power objective (HPO)

Microscopy and Magnification: Study Notes for General Biology

Study Guide - Practice Questions

Study Guide - Flashcards